HO-ED-S-06B Confocal Laser Raman Spectrometer

Regular price $32,175.00

Confocal Laser Raman Spectrometer

Holmarc's Confocal Laser Raman Spectrometer couples a Raman spectrometer to a standard optical microscope, allowing highly magnified visualization of a sample and Raman analysis with a microscopic laser spot. In this equipment, sample is placed on the top of the microscope (Inverse confocal method)and is focused and then the measurement is taken. We can also analyze the sample output intensity vs. Raman shift spectrum which is connected via USB on a computer screen.

Confocal means having the same focus. Unlike a normal microscope, where the whole sample is illuminated, the confocal microscope only measures the light from a small volume in the sample at any given time. Raman imaging, which combines Raman spectroscopy and imaging techniques, is an excellent method for obtaining information about the spatial distribution of molecular species. The principle on which Raman Imaging is based, is that the spatial distribution of a molecular species is obtained by isolating the Raman scattered photons from a Raman line of the specific molecular species. Raman Imaging is a 3-dimensional problem (1 spectral and 2 spatial dimensions). In Confocal microscopy we use series-imaging method in which, point illumination technique is used. A laser spot is scanned across the sample. At each resolved position, the light is collected, spectrally filtered and detected by a detector. Confocal Raman microscopy refers to the ability to spatially filter the analysis volume of the sample, in the XY (lateral) and Z (depth) axes.

Experiment:

  •     To record the Raman spectrum of Solid and liquid samples using spectrometer
  •     To visualize the live mode of molecule in action and its Studies

Specifications:

 Design     Czerny Turner design
    Type     Plane grating
    Lines / mm     1200 l/m
    Wavelength range     538 nm to 598 nm *
    Raman Shift range     210 cm-1 -  2000 cm-1
    Wavelength accuracy     < 0.4 nm
    Wavelength repeatability     < 0.2 nm
    Laser     532nm, 40mW DPSS laser
    Notch filter wavelength     532 nm
    CWL tolerance (nm)     +/- 5.3 nm
    Calibration source used     Mercury lamp
    Calibration type     Regression / curve fitting
    * (other wavelength ranges are available on request)

CCD:

  CCD detector     High sensitive CCD detector 3648 element
    Signal to noise ratio     500 : 1
    A/D resolution     12 / 16 Bit
    Exposure time     0.1 - 6500 ms

Features:

  Computer-controlled, user-friendly interface
    Automatic recording of Raman spectra
    DPSS laser is used as light source
    Both solid and liquid samples can be analyzed
    System is assembled as standalone unit

Optional Accessories:

1. Sample Heating Unit
        Model A : Temperature Range : RT - 200º C
        Model B : Temperature Range : 200 - 482º C
    2. Specimen Holders
        Multiwell Plate Holder
        Petri Dish Holder
    3. Motorized objective lens selection unit

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