Confocal Laser Raman Spectrometer
Holmarc's Confocal Laser Raman Spectrometer couples a Raman spectrometer to a standard optical microscope, allowing highly magnified visualization of a sample and Raman analysis with a microscopic laser spot. In this equipment, sample is placed on the top of the microscope (Inverse confocal method)and is focused and then the measurement is taken. We can also analyze the sample output intensity vs. Raman shift spectrum which is connected via USB on a computer screen.
Confocal means having the same focus. Unlike a normal microscope, where the whole sample is illuminated, the confocal microscope only measures the light from a small volume in the sample at any given time. Raman imaging, which combines Raman spectroscopy and imaging techniques, is an excellent method for obtaining information about the spatial distribution of molecular species. The principle on which Raman Imaging is based, is that the spatial distribution of a molecular species is obtained by isolating the Raman scattered photons from a Raman line of the specific molecular species. Raman Imaging is a 3-dimensional problem (1 spectral and 2 spatial dimensions). In Confocal microscopy we use series-imaging method in which, point illumination technique is used. A laser spot is scanned across the sample. At each resolved position, the light is collected, spectrally filtered and detected by a detector. Confocal Raman microscopy refers to the ability to spatially filter the analysis volume of the sample, in the XY (lateral) and Z (depth) axes.
Experiment:
-
To record the Raman spectrum of Solid and liquid samples using spectrometer
-
To visualize the live mode of molecule in action and its Studies
Specifications:
Design |
Czerny Turner design |
Type |
Plane grating |
Lines / mm |
1200 l/m |
Wavelength range |
538 nm to 598 nm *
|
Raman Shift range |
210 cm-1 - 2000 cm-1
|
Wavelength accuracy |
< 0.4 nm |
Wavelength repeatability |
< 0.2 nm |
Laser |
532nm, 40mW DPSS laser |
Notch filter wavelength |
532 nm |
CWL tolerance (nm) |
+/- 5.3 nm |
Calibration source used |
Mercury lamp |
Calibration type |
Regression / curve fitting |
* (other wavelength ranges are available on request)
|
CCD:
CCD detector |
High sensitive CCD detector 3648 element |
Signal to noise ratio |
500 : 1 |
A/D resolution |
12 / 16 Bit |
Exposure time |
0.1 - 6500 ms |
Features:
Computer-controlled, user-friendly interface |
Automatic recording of Raman spectra |
DPSS laser is used as light source |
Both solid and liquid samples can be analyzed |
System is assembled as standalone unit |
Optional Accessories:
1. Sample Heating Unit |
Model A : Temperature Range : RT - 200º C |
Model B : Temperature Range : 200 - 482º C |
2. Specimen Holders |
Multiwell Plate Holder |
Petri Dish Holder |
3. Motorized objective lens selection unit |
Graph:
